Multiplication of Dioscorea alata clone Chino Blanco in temporary immersion systems (Original)
Keywords:
bioreactors; tissue culture; micropropagation; yamAbstract
Yam is a crop of high nutritional value for the population, but the insufficient availability of planting material limits its cultivation on a large scale. Through the use of biotechnological tools, its propagation is possible, although there are few works that use Temporary Immersion Systems. The objective of the work was to evaluate the effect of different times and frequencies of temporal immersion, on the multiplication of nodal segments in Dioscorea alata clone Chino Blanco. First, three immersion times (5.0, 10.0 and 15.0 minutes) were evaluated with a frequency of every eight hours. Then the best time was set and the response was evaluated with immersion frequencies every 4, 6 and 8 hours per day. At 21 days of cultivation, the color of the shoots, the number of shoots, the length of the shoots and the multiplication coefficient were evaluated. In summary, the multiplication of the nodal segments of the Chino Blanco clone yam was achieved, with significant differences between the treatments used in the SETIS™ temporary immersion systems. With the use of ten minutes of immersion every six hours, the highest number of nodal segments was obtained (3.85), the average length of the shoots increased (1.68 cm) and no symptoms of hyperhydricity were observed during the 21 days of cultivation.
References
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Niemenak, N., Mboene Noah, A., & Ndoumou Omokolo, D. (2013). Micropropagation of cocoyam (Xanthosoma sagittifolium L. Schott) in temporary immersion bioreactor. Plant Biotechnology Reports, 7(3), 383–390. https://doi.org/10.1007/s11816-013-0272-5
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Salazar, R., & Hoyos, R. A. (2007). Multiplicación y tuberización in vitro de ñame (Dioscorea alata L.) en sistema de inmersión temporal. Revista Facultad Nacional de Agronomía Medellín, 60(2), 3907-3921. https://revistas.unal.edu.co/index.php/refame/article/view/24418
Sánchez, L. A., Labrada, E. D., & Borges, M. (2019). Evaluación morfológica de plantas de ñame (Dioscorea spp.) procedentes de tubérculos sanos a los cuatro y siete meses de edad. Redel. Revista Granmense De Desarrollo Local, 3(1), 138-145. https://revistas.udg.co.cu/index.php/redel/article/view/686
Valdés, E. (2019) Caracterización de potyvirus en Dioscorea spp. en el Banco de Germoplasma de ñame del INIVIT [Tesis de Diploma, Universidad Central “Martha Abreu” de Las Villas], Santa Clara, Villa Clara, Cuba. https://dspace.uclv.edu.cu/handle/123456789/11407
Yan, H., Yang, L., & Li, Y. (2011). Improved growth and quality of Dioscorea fordii Prain et Burk and Dioscorea alata plantlets using a temporary immersion system. African Journal of Biotechnology, 10(83), 19444-19448. https://www.ajol.info/index.php/ajb/article/view/99066
Bahí, M., & Pérez, J. L. (2021). Multiplicación de brotes de morera variedad Doña Betty en Sistema de Inmersión Temporal. Redel. Revista Granmense de Desarrollo Local, 5(1), 64-73. https://revistas.udg.co.cu/index.php/redel/article/view/2157
Balogun, M., Maroya, N., Asiedu, R., & Taiwo, J. (2014). Novelty, rapidity and quality in seed yam production: The case of Temporary Immersion Bioreactors. IITA.
Balogun, M., Maroya, N., Taiwo, J., Chukwunalu, O., Ajayi, A., Kumar, P. L., Pelemo, O., Aighewi, B., & Asiedu, R. (2017). Clean Breeder Seed Yam Tuber Production using Temporary Immersion Bioreactors. IITA.
Cabrera, M., Basail, M., Torres, Y., Robaina, A., Santos, A., Medero, V., Rayas, A., López, J., García, M., Ventura, J., & Oliva, M. (2004). Empleo de Sistemas de Inmersión Temporal para la multiplicación de segmentos nodales de Dioscorea alata L. en el clon ‘Pacala Duclos’. Biotecnología Vegetal, 4(1), 3-8. https://revista.ibp.co.cu/index.php/BV/article/view/293
Cabrera, M., Gómez, R., Rodríguez, S., López, J., Rayas, A., Basail, M., Santos, A., Medero, V., & Rodríguez, G. (2008). Multiplicación in vitro de segmentos nodales del clon de ñame Blanco de Guinea (Dioscorea cayenensis-D. rotundata) en sistemas de cultivo semiautomatizado. Revista Colombiana de Biotecnología, 10(2), 97-103. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/9182
Cabrera, M., Gómez, R., Rayas, A., De Feria, M., López, J., Basail, M., & Medero, V. (2009). Protocolo para la formación de microtubérculos de ñame (Dioscorea alata L.) en sistema de inmersión temporal. Revista Colombiana de Biotecnología, 11(2), 19-30. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/11678
Cabrera, M., Gómez, R., Espinosa, E., & Espinosa, A. (2012). Efficiency of semi-automated culture systems on microtubers formation of yam (Dioscorea alata L.). Biotechnologie, Agronomie, Société et Environnement, 16(1), 45-47. https://popups.uliege.be/1780-4507/index.php?id=8373
Chukwunalu, O., Balogun, M., Maroya, N., & Asiedu, R. (2018). Development of micropropagation system for yam (Dioscorea spp.) using somatic embryogenesis. IITA. https://biblio1.iita.org/handle/20.500.12478/5521
Escalona, M., Lorenzo, J., González, B., Daquinta, M., Barroto, C., González, J., & Desjardines, Y. (1999). Pineapple (Annanas comosus Merr) micropropagation in temporary immersion systems. Plant Cell Reports, 18(9), 743-748. https://doi.org/10.1007/s002990050653
Etienne, H., & Berthouly, M. (2002). Temporary immersion systems in plant micropropagation. Plant Cell, Tissue and Organ Culture, 69(3), 215-231. https://doi.org/10.1023/A:1015668610465
Gonzaga, L., López, R., & Morales, T. (2016). Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA®. African Journal of Biotechnology, 15(28), 1503-1510. https://doi.org/10.5897/AJB2016.15390
González, M. E. (2012). El ñame (Dioscorea sp.): Características, usos y valor medicinal. Aspectos de importancia en el desarrollo de su cultivo. Cultivos Tropicales, 33(4), 5-15. https://ediciones.inca.edu.cu/index.php/ediciones/article/view/184
Hussein I., Mengs, B., & Matiwos, T. (2018). Effect of plant growth regulators on in vitro propagation of yam Landraces (Dioscorea Species) using nodal segments. Journal of Biology, Agriculture and Healthcare, 8(21), 13-23. https://core.ac.uk/download/pdf/234662712.pdf
Iseki, K., & Matsumoto, R. (2020). Effect of seed sett size on sprouting, shoot growth, and tuber yield of white guinea yam (Dioscorea rotundata). Plant Production Science, 23(1), 75–80. https://doi.org/10.1080/1343943X.2019.1667835
Mancilla, E., Pérez, J. A., Núñez, R., Spinoso, J. L., & Bello, J. J. (2021). Comparison of different Semi-Automated Bioreactors for in vitro propagation of taro (Colocasia esculenta L. Schott). Plants, 10, 1010. https://doi.org/10.3390/plants10051010
Niemenak, N., Mboene Noah, A., & Ndoumou Omokolo, D. (2013). Micropropagation of cocoyam (Xanthosoma sagittifolium L. Schott) in temporary immersion bioreactor. Plant Biotechnology Reports, 7(3), 383–390. https://doi.org/10.1007/s11816-013-0272-5
Polzin, F., Sylvestre, I., Déchamp, E., Ilbert, P., Etienne, H., & Engelmann, F. (2014). Effect of activated charcoal on multiplication of African yam (Dioscorea cayenensis-rotundata) nodal segments using a temporary immersion bioreactor (RITA®). In Vitro Cellular & Developmental Biology-Plant, 50(2), 210-216. https://www.researchgate.net/profile/F-Engelmann/publication/263245437_Effect_of_activated_charcoal_on_multiplication_of_African_yam_Dioscorea_cayenensis-rotundata_nodal_segments_using_a_temporary_immersion_bioreactor_RITA_R/links/55b7609608ae092e965712d9/Effect-of-activated-charcoal-on-multiplication-of-African-yam-Dioscorea-cayenensis-rotundata-nodal-segments-using-a-temporary-immersion-bioreactor-RITA-R.pdf
Salazar, R., & Hoyos, R. A. (2007). Multiplicación y tuberización in vitro de ñame (Dioscorea alata L.) en sistema de inmersión temporal. Revista Facultad Nacional de Agronomía Medellín, 60(2), 3907-3921. https://revistas.unal.edu.co/index.php/refame/article/view/24418
Sánchez, L. A., Labrada, E. D., & Borges, M. (2019). Evaluación morfológica de plantas de ñame (Dioscorea spp.) procedentes de tubérculos sanos a los cuatro y siete meses de edad. Redel. Revista Granmense De Desarrollo Local, 3(1), 138-145. https://revistas.udg.co.cu/index.php/redel/article/view/686
Valdés, E. (2019) Caracterización de potyvirus en Dioscorea spp. en el Banco de Germoplasma de ñame del INIVIT [Tesis de Diploma, Universidad Central “Martha Abreu” de Las Villas], Santa Clara, Villa Clara, Cuba. https://dspace.uclv.edu.cu/handle/123456789/11407
Yan, H., Yang, L., & Li, Y. (2011). Improved growth and quality of Dioscorea fordii Prain et Burk and Dioscorea alata plantlets using a temporary immersion system. African Journal of Biotechnology, 10(83), 19444-19448. https://www.ajol.info/index.php/ajb/article/view/99066
Published
2022-07-08
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How to Cite
Multiplication of Dioscorea alata clone Chino Blanco in temporary immersion systems (Original). (2022). REDEL. Revista Granmense De Desarrollo Local, 6(2), 234-252. https://revistas.udg.co.cu/index.php/redel/article/view/3428
