Multiplication of Dioscorea alata clone Chino Blanco in temporary immersion systems (Original)
Keywords:
bioreactors; tissue culture; micropropagation; yamAbstract
Yam is a crop of high nutritional value for the population, but the insufficient availability of planting material limits its cultivation on a large scale. Through the use of biotechnological tools, its propagation is possible, although there are few works that use Temporary Immersion Systems. The objective of the work was to evaluate the effect of different times and frequencies of temporal immersion, on the multiplication of nodal segments in Dioscorea alata clone Chino Blanco. First, three immersion times (5.0, 10.0 and 15.0 minutes) were evaluated with a frequency of every eight hours. Then the best time was set and the response was evaluated with immersion frequencies every 4, 6 and 8 hours per day. At 21 days of cultivation, the color of the shoots, the number of shoots, the length of the shoots and the multiplication coefficient were evaluated. In summary, the multiplication of the nodal segments of the Chino Blanco clone yam was achieved, with significant differences between the treatments used in the SETIS™ temporary immersion systems. With the use of ten minutes of immersion every six hours, the highest number of nodal segments was obtained (3.85), the average length of the shoots increased (1.68 cm) and no symptoms of hyperhydricity were observed during the 21 days of cultivation.
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