Acclimatization in vitro plant cultivation of Morusalba variety Acorazonada (Original)

Abstract

Research works have been carried out for the in vitro propagation of mulberry; however, there is low survival of in vitro plants in the acclimatization phase. The objective of the research was to increase the survival and quality of the in vitro plants of mulberry variety Acorazonada during the acclimatization phase. Substrates mixtures were evaluated: 1) Cambisol soil 100 % (control), 2) earthworm humus 85 % + zeolite 15 %, 3) bovine manure 85 % + zeolite 15 %, 4) sheep manure 85 % + zeolite 15%; then the in vitro plants were classified according to size ranges: 1): 1.0-3.0 cm; 2): 3.1-5.0 cm; 3): 5.1-7.0 cm). A completely randomized experimental design and Tukey test (p≤0.05) were used. With the use of substrate mixture two and the use of plants with sizes from 5.1 to 7.0 cm, a survival 85 % was obtained; in addition the height of the plant, number, length and width was increased of the leaves, without significant differences with respect to treatment three during the acclimatization of plants from in vitro culture.