In vitro multiplication of banana clon INIVIT PV 06-30 (Musa spp) in biofabricas conditions (Original)

Abstract

The improvement of the methodologies for in vitro multiplication of crops is a constant need in productive processes developed in biofactories. The influence of the physical status, macroelements's concentration in culture medium and the subcultures number were evaluate to in vitro multiplication of banana INIVIT PV 06-30 clon’s in biofactory conditions. In vitro plants from meristems were using as plant material. Three concentrations (100 %, 75 % and 50 %) of the macroelement’s included in Murashigue y Skoog (1962) culture medium were evaluated in combination with two physical status of the culture medium (semisolid and liquid) during six subcultures. The results show higher values of coefficient of multiplication, length of the shoot and length and width of the leaves in the culture medium with the macroelements at 100 % to their concentration, regardless of the physical state of the culture medium. The coefficient of multiplication was low in all subculture, without differences from the second.