Multiplication of mulberry shoots variety Doña Betty in Temporary Immersion System (Original)
Keywords:
bioreactors; in vitro culture; spread; SETIS ™Abstract
Mulberry (Morus alba L.) is a forage plant native to the Himalayas, which has shown excellent qualities for feeding different species of animals, whose nutritional value is one of the highest among non-legume tropical forages. Studies carried out in Cuba have shown that mulberry is an alternative food that allows the partial substitution of commercial concentrates in the diet of monogastrics and ruminants. An alternative to conventional propagation methods is the use of in vitro plant tissue culture techniques; however there are few works that use Temporary Immersion Systems. The objective of the research was to determine the time and frequency of immersion for the multiplication of shoots of mulberry variety Doña Betty in the SETIS ™ Temporary Immersion System. Treatments with immersion times of 1.0; 2.0 and 3.0 minutes of duration with frequencies every four, six and eight hours daily; Three SETIS ™ bioreactors were used for each treatment, with 30 explants per flask. At 28 days of culture, the number of shoots, length of the shoot, number of nodal segments and number of leaves were determined. As a result, it was possible to increase the multiplication and quality of the Doña Betty variety mulberry shoots, employing two minutes of immersion every eight hours in a temporary immersion system.
References
Carvalho, L.S.O., Ozudogru, E.A., Lambardi, M. y Paiva, L.V. (2019). Temporary Immersion System for Micropropagation of Tree Species: a Bibliographic and Systematic Review. NotulaeBotanicaeHortiAgrobotanici, 47 (2): 269-277.
Georgiev, V., Schumann, A., Pavlov, A. y Bley, T. (2014). Temporary immersion systems in plant biotechnology. Engineering in Life Science, 14: 607–62.
Gianguzzi, V.,Inglese, P.,Barone, E. y Sottile, F. (2019). In vitro regeneration of Capparisspinosa L. by using a temporary immersion system. Plants, 8: 177.
Hesami, M., Daneshvar, M. y Yoosefzadeh-Najafabadi M. (2019). An efficientin vitro shoot regeneration through direct organogenesis from seedling-derived petiole and leaf segments and acclimatization of Ficusreligiosa. J. Forest. Res., 30(3): 807–815.
Martín, G.J., Montejo, J.I., Milera, M.C. y García, D.E. (2017). Chapter 2. Chemical composition and nutritive value of mulberry (Morus alba) in animal feeding. En: Mulberry, moringa and tithonia in animal feed, and other uses. Results in Latin America and the Caribbean, (eds) L.L Savon, O. Gutierrez, G. Febles, (Ed.) FAO-ICA, Cuba, pp: 29-42.
Mendonça, E.G., Stein, V.C., Carvalho, H.H., Santos, B.R., Beijo, L.A. y Paiva, L.V. (2016). The use of continuous, temporary immersion bioreactor system and semisolid culture medium for the production of Eucalyptus camaldulensis clones. CiênciaFlorestal, 26 (4): 1211-1224.
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco cultures. PhysiolPlant, 15 (3): 473-479.
Rocano, M.N., Villena, P.G. y Peña, D.F. (2017). Evaluación de los sistemas de cultivo semisólido y BIT en la multiplicación in vitro de Juglans neotrópica. Maskana, 8 (1): 103-109.
Rosales, C., Brenes, J., Salas, K., Arceo-Solano, S.yAbdelnour-Esquivel, A. (2018). Micropropagación de Steviarebaudiana en sistemas de inmersión temporal para incursionar en la producción hortícola. Revista Chapingo Serie Horticultura, 24(1): 69-84.
Salas, E., Agramonte, D., Jiménez-Terry, F., Pérez, M., Collado, R., Barbón, R. La O, M., De Feria, M. y Chávez, M. (2011). Propagación de plantas de Morus alba var. Criolla con el uso de sistemas de inmersión temporal. Biotecnología Vegetal 11 (2): 77-88.
Silva, J.A., Solis-Gracia, N., Jifon, J., Creste, S. y Kiran, K. (2020). Use of bioreactors for large-scale multiplication of sugarcane (Saccharum spp.), energy cane (Saccharum spp.), and related species. In Vitro Cellular & Developmental Biology-Plant, 56: 366-376.
Vervit. (2016). SETIS. En: http://www.setis-systems.be/SETISsystems/About_ SETIS.html.
Vidal, N. y Sánchez, C. (2019). Use of bioreactor systems in the propagation of forest trees. Engineering in Life Sciences, 19: 896–915.
