Propagation and in vitro conservation of carnation (Dianthus ssp.) (Original)

Abstract

The carnation is one of the main species that produces cut flowers internationally. In this research, three experiments were carried out aimed at evaluating the propagation and in vitro conservation of different varieties of carnation with the use of culture means. In a first experiment, disinfection with 1% sodium hypochlorite of active chlorine was carried out for 20 minutes in the laminar flow booth and then they were washed with sterile distilled water 4 times and later they were seeded in the establishment medium with the Murashige-Skoog salts (1962) supplemented with myinositol 100 mg.l-1, thiamine 1 mg.l-1, gibberellic acid 10 mg. l-1, indolacetic acid 0,01 mg.l-1, sucrose 30 g.l-1, agar 6 g. l-1 and pH 5,7. The five evaluated varieties of Chinese carnation presented a better response to disinfection and to the variables of the in vitro establishment than the Spanish carnation varieties. In a second experiment in the multiplication stage using the previous culture medium, five varieties of Chinese carnation were compared in terms of the percentage of sprouting and rooting, as well as the number of nodal segments at 28 days of culture. The MR and RF varieties presented the highest number of nodal segments with values of 7,37 and 6,92 respectively and without significant differences between them. In a third experiment, a trial was carried out with the objective of evaluating the possibility of conserving in vitro material of the Spanish carnation (Dianthus caryophillus) in ¨bottle¨ type containers for the conservation of in vitro material. The segments were seeded in the carnation multiplication environment. In vitro conservation of Spanish carnation was achieved in bottles type “bottle” for one year.