Evaluation of the antibacterial activity of molasses against Vibrio sp. isolated from culture ponds of Litopenaeus vannamei (shrimp) (Original)
Keywords:
molasses; antimicrobial activity; Vibrio; Litopenaeus vannameiAbstract
Strains of Vibrio sp were isolated from ponds in preparation for the sowing of shrimp postlarvae Litopenaeus vannamei, in the shrimp farm of the South Coast of Granma (Calisur). The water samples were taken before and after the application of molasses, in them total Vibrio levels were observed in the order of 102. The positive sucrose Vibrio (yellow colonies) predominated with respect to the negative sucrose Vibrio (green colonies). For the evaluation of the antimicrobial activity was used the Bauer-Kirby disc diffusion agar method with modifications. The molasses not showed antibacterial activity against Vibrio sp, although the stains were sensitive to gentamicin and extremely sensitive to ciprofloxacin, antibiotics used to counteract bacterial infections in shrimp farms.
References
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12. Gutiérrez, E. (1995). Efecto de los ácidos grasos volátiles del proceso resumen abomasal (in vitro) y de la melaza, sobre la viabilidad de Salmonella typhimurium (tesis doctoral). Universidad de Colima, Colima, México.
13. Heenatigala, P.P.M., y Fernando, M.U.L. (2016). Occurrence of bacteria species responsible for vibriosis in shrimp pond culture systems in Sri Lanka and assessment of the suitable control measures. Sri Lanka J Aquatic Science, 21(1), 1-17.
14. Kongchuma, P., Chimtonga, S., Chareansaka, N., y Subpraserta, P. (2016). Effect of Green Tea ExtractonVibrio parahaemolyticus Inhibition in Pacific White Shrimp (Litopenaeus vannamei) Postlarvae. Agriculture and Agricultural Science Procedia, 11(9), 117–124.
15. Luna, A., Ávila, J., Fierro, J.A., Álvarez, P., Esparza, H., Escamilla, R., Flores, M.C., Montiel, J., y López, E.S. (2017). Effects of bacilli, molasses, and reducing feeding rate on biofloc formation, growth, and gene expression in Litopenaeus vannamei cultured with zero water exchange. Lat. Am. J. Aquat. Res, 45(5), 900-907.
2. Bauer, A.W., Kirby, W.M.M., Sherris, J.C., y Truck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(6), 493-496.
3. Bermúdez, M.C., Espinosa, A., Santiago, M.L., Barajas, C.J., y Acedo, E. (2014). Comportamiento de oxitetraciclina en camarón de cultivo Litopenaeus vannamei y la sensibilidad a tres antibióticos de bacterias de Vibrio aisladas de los organismos. Revista de Ciencias Biológicas y de la Salud, 16(3), 29-37.
4. Castro, M. (1993). Estudio de la melaza de caña como sustrato de la fermentación acetobutílica (tesis de pregrado). Universidad Nacional de Colombia, Bogotá, Colombia.
5. Celikel, N., y Kavas, G. (2008). Antimicrobial properties of some essential oils against some pathogenic microorganisms. Czech Journal of Food Science, 26(3), 174-181.
6. Chatterjee, S., y Haldar, S. (2012). Vibrio Related Diseases in Aquaculture and Development of Rapid and Accurate Identification Methods. J Marine Sci Res Dev, 1(2), 73-90.
7. Citarasu, T. (2010). Herbal biomedicines: a new opportunity for aquaculture industry. Aquaculture International, 18(3), 403-414.
8. Clinical and Laboratory Standards Institute. (2009). Performance Standards for Antimicrobial Disk Susceptibility Tests (M02- A10)
9. Fuentes, E.A., y Guillén, G.M. (2014). Utilización de melaza como fertilizante orgánico de estanques camaroneros durante la fase de engorde del camarón marino (Litopenaeus vannamei) (tesis de pregrado). Universidad de El salvador, San Salvador, El Salvador.
10. Gracia, M.H., Ávila, L.A., Yepiz, G., Hernández, J., Mendoza, F., García, G., y Gollas, T. (2011). Assessing the viability of necrotizing hepatopancreatitis bacterium (NHPB) stored at −20 °C for use in forced-feeding infection of Penaeus (Litopenaeus vannamei). Aquaculture, 31(4), 105-109.
11. Gracia, M.H., Orozco, C., y Molina, C. (2012). Efecto antibacteriano del aceite esencial de orégano (Lippia berlandieri) en bacterias patógenas de camarón (Litopenaeus vannamei). Hidrobiológica, 22(3), 201-206.
12. Gutiérrez, E. (1995). Efecto de los ácidos grasos volátiles del proceso resumen abomasal (in vitro) y de la melaza, sobre la viabilidad de Salmonella typhimurium (tesis doctoral). Universidad de Colima, Colima, México.
13. Heenatigala, P.P.M., y Fernando, M.U.L. (2016). Occurrence of bacteria species responsible for vibriosis in shrimp pond culture systems in Sri Lanka and assessment of the suitable control measures. Sri Lanka J Aquatic Science, 21(1), 1-17.
14. Kongchuma, P., Chimtonga, S., Chareansaka, N., y Subpraserta, P. (2016). Effect of Green Tea ExtractonVibrio parahaemolyticus Inhibition in Pacific White Shrimp (Litopenaeus vannamei) Postlarvae. Agriculture and Agricultural Science Procedia, 11(9), 117–124.
15. Luna, A., Ávila, J., Fierro, J.A., Álvarez, P., Esparza, H., Escamilla, R., Flores, M.C., Montiel, J., y López, E.S. (2017). Effects of bacilli, molasses, and reducing feeding rate on biofloc formation, growth, and gene expression in Litopenaeus vannamei cultured with zero water exchange. Lat. Am. J. Aquat. Res, 45(5), 900-907.
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2019-03-05
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Evaluation of the antibacterial activity of molasses against Vibrio sp. isolated from culture ponds of Litopenaeus vannamei (shrimp) (Original). (2019). REDEL. Revista Granmense De Desarrollo Local, 3(1), 48-57. https://revistas.udg.co.cu/index.php/redel/article/view/679
