Effect of the state of development of the cotyledons in the induction of soya somatic embryos (Original)
Keywords:
Glycinemax; histology; regeneration; somaticembryogenesisAbstract
Somatic embryogenesis is a response of cell totipotency in plants that allows plants to be obtained without the intervention of gametes. This morphogenetic event has been described in different plant species, but its response has been limited in different genotypes of legume species such as soybeans. The objective of the work was to evaluate the effect of the development status of cotyledons in the embryogenic response in soybean cultivar Incasoy-27. Immature cotyledons from 2.0 to 6.0 mm in length were used, which were placed in somatic embryo formation culture medium containing 40 mg.L-1 of 2,4-D as a growth regulator for 28 days. The number of cotyledons with embryogenic response was determined and the number of somatic embryos formed by cotyledon. Histological sections were also performed on cotyledons of 3.0 and 6.0 mm in length, cultured for six days in the presence of 2,4-D. As a result, the formation of somatic embryos was obtained from immature cotyledons with significant differences between different lengths evaluated. Cotyledons with 4.0 mm length had the highest proliferation of embryogenic cells (92.0%) and the formation of about eight somatic embryos. Histological analyzes showed that the development status of the cotyledons and their cellular composition influenced the embryogenic response, fundamentally on the adaxial side of the cotyledons.
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